Citing Us

If you used the High Resolution Macromolecular Electron Microscopy facility, we ask that you acknowledge us in all publications, using the following text:

Grids were prepared and data collected at the High Resolution Macromolecular Electron Microscopy (HRMEM) facility at the University of British Columbia (https://cryoem.med.ubc.ca). We thank Claire Atkinson, Amy Wo, Barathy Deivanayaga, Liam Worrall and Natalie Strynadka. HRMEM is funded by the Canadian Foundation for Innovation and the British Columbia Knowledge Development Fund.

Users should also feel free to use the following templates for the Methods section of their publications.

Vitrification

Grids were frozen at the UBC Facility for High-Resolution Macromolecular Cryo-EM using an Vitrobot Mark IV (Thermo Fisher) with blot force X, blot time Xs, X°C and X% humidity.

 

Negative stain

Images were collected at the UBC Facility for High-Resolution Macromolecular Cryo-EM on a 120kV Talos microscope (Thermo Fisher) equipped with a CETA camera (Thermo Fisher) using TIA software. The magnification was Xkx, with a pixel size of XA2. The dose was Xe-/A2, and the images were taken with a Xs exposure time and a nominal defocus of -Xum.

 

Screening

Grids were screened at the UBC Facility for High-Resolution Macromolecular Cryo-EM on a 200kV Glacios microscope (Thermo Fisher) equipped with a Falcon 3 camera (Thermo Fisher) to determine suitability for further data collection.

 

Data collection

Data were collected at the UBC Facility for High-Resolution Macromolecular Cryo-EM on a 300kV Krios G2 microscope (Thermo Fisher) equipped with a Falcon 4i camera (Thermo Fisher) and Selectris energy filter (Thermo Fisher), using EPU software (Thermo Fisher). Data were collected at Xx magnification with a pixel size of XA and a total dose of Xe-/A2.

 

Microscopy for LNPs

Images were collected at the UBC Facility for High-Resolution Macromolecular Cryo-EM on a 200kV Glacios microscope (Thermo Fisher) equipped with a Falcon 3 camera (Thermo Fisher) using TIA software. The magnification was 73kx, with a pixel size of 1.98A2. The dose was Xe-/A2, and the images were taken with a 3s exposure time and a nominal defocus of -2.5um

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