Users must supply their own grids and grid boxes

Users are responsible for storing their own grids

Users must supply a hard drive for transferring data

All hard drives (unless brand new in packaging) will be reformatted by the facility staff prior to data transfer

Data MUST be transferred in a timely manner

Once data has been transferred the facility will no longer bear responsibility for storing the data- PLEASE make sure you keep a redundant backup of all your data as we assume no responsibility for data loss in the event of data workflow issues

Users are responsible for analyzing their own data

The facility does not currently provide analysis workstations, please do not use the computers in the microscope control room for your data analysis

Facility staff cannot analyse data on behalf of users, but are available for questions and consultation

We recommend these computing resources

We recommend these analysis software packages

Before users are allowed to schedule bookings they must demonstrate their project is appropriate for cryo-EM using the sample proposal form

Booking of screening sessions is prioritised by project feasibility

The priority for the Glacios is screening and exploratory data collection; the priority for the Krios is multi day data collection

Data collection sessions will be scheduled on a priority basis depending on project feasibility, quality of grids, initial data, and wait time

24 hour data collection is inclusive of set up time and runs from 10.30am to 10am the following day

Overnight data collection runs until 10am the following day

Each lab may only have one weekend collection booked at any one time

Weekend collections must involve samples that can be collected all weekend with no additional input from operators

For full day and weekend data collections, grids must be pre-approved

There should be a backup for saved grids, and in the case of grid loss a penalty for not going forward due to lack of sample will be charged

Vitrobot use is scheduled on a first-come, first-served basis after project approval

The Vitrobot is available for half-day sessions, either 9 a.m. - 12 p.m. or 1 p.m. - 4 p.m.

You finish using the Vitrobot by the end of your booked session, or you will be charged for 2 sessions

All training must be done by facility staff

Preparation and loading of autogrids will be done by facility staff, not users

Staff will designate users as super-users when they are satisfied the user can operate the microscope software without supervision

Users must undergo training from facility staff in order to independently use the Vitrobot

Users must undergo training from staff in order to set up data collections and screen grids independently

Trained super-users can set up data collection and carry out screening on behalf of other members of their group but are NOT permitted to train other users.

Users may not enter the Glacios and Krios microscope rooms

Appropriate PPE must be worn when handling liquid ethane and liquid nitrogen

No food or drink in the Talos room or sample prep room

If working alone, users must notify another person of their whereabouts

If there are issues with the equipment the user should notify Claire immediately

At no point should a user attempt to fix equipment by themselves