New User Guide

1.  Consultation
   • Email ceatk@mail.ubc.ca to discuss your experiment and whether the facility can provide appropriate services for your needs.
2.  Submit billing information form to Suzette de Jesus
   • Internal users should submit a signed billing information form to sdejesus@mail.ubc.ca, telling her that it is for the HRMEM facility.
3. Register with iLab to be able to view the instrument schedules
4. Buy the supplies you will need
   • Please see our ‘What to Bring’ page for the consumables we request the user supplies.
5.  Get trained! We offer:
   • Screening training in negative stain on the Talos 120 TEM
     • Schedule training to look at your samples via negative stain imaging, often the first validation of appropriate sample quality.
     • During these sessions, you can work towards being authorized to screen grids independently.
     • It may take multiple screening sessions to find a good condition for each sample.
   • Vitrobot training
     • Schedule a training session to learn how to use the Vitrobot.
     • We can freeze samples for you but we recommend that regular users undergo training to enable independent use.
     • Freeze up to 11 grids for screening.

   • CryoEM screening of vitrified grids on the Glacios 200 TEM

     • Schedule a screening session to assess ice and particle quality on your vitrified grid.

     • Performed by facility staff, but users are welcomed and encouraged to shadow and learn about grid features and what is required for subsequent high resolution data collection, or if needed, further sample optimization.

   •  High resolution Data collection/ EPU training on the Krios 300 TEM
     • Schedule an overnight data collection session.
     • Setup for data collection is carried out by facility staff using the EPU software for automated data collection. Users are encouraged and welcomed to shadow to learn about data collection parameters and strategies appropriate to their sample.
   •  Data Processing and Analysis
     • Users generally process and analyse the larger data sets themselves, although the facility can provide expertise via paid consultation.
     • We also provide resources on our website for support and tutorials, and are happy to answer questions.

Please follow the link below our page below for more information about the project workflow: