It is critical to our ongoing funding that acknowledgement of HRMEM’s contributions to your research are made in all publications, posters and talks.
We ask that you acknowledge HRMEM using the following statements:
Grids were prepared and data collected at the High Resolution Macromolecular Electron Microscopy (HRMEM) facility at the University of British Columbia (https://cryoem.med.ubc.ca). We thank Claire Atkinson, Amy Wo, Barathy Deivanayaga, Liam Worrall and Natalie Strynadka. HRMEM is funded by the Canadian Foundation for Innovation and the British Columbia Knowledge Development Fund.
Users are also encouraged to use the following templates for the Methods section of their publications.
Vitrification
Grids were frozen at the UBC High-Resolution Macromolecular Cryo-EM (HRMEM) facility using a Vitrobot Mark IV (Thermo Fisher) with blot force of X, blot time of Xs, X°C and X% humidity.
Negative stain
Images were collected at the UBC High-Resolution Macromolecular Cryo-EM (HRMEM) facility on a 120kV Talos TEM microscope (Thermo Fisher) equipped with a CETA camera (Thermo Fisher) using TIA software. The magnification was Xkx, with a pixel size of XA2. The dose was Xe-/A2, and the images were taken with a Xs exposure time and a nominal defocus of -Xum.
Screening
Grids were screened at the UBC High-Resolution Macromolecular Cryo-EM (HRMEM) facility on a 200kV Glacios microscope (Thermo Fisher) equipped with a Falcon 3 camera (Thermo Fisher) to determine suitability for further data collection.
Data collection
Data were collected at the UBC High-Resolution Macromolecular Cryo-EM (HRMEM) facility on a 300kV Krios G2 microscope (Thermo Fisher) equipped with a Falcon 4i camera (Thermo Fisher) and Selectris energy filter (Thermo Fisher), using EPU software (Thermo Fisher). Data were collected at Xx magnification with a pixel size of XA and a total dose of Xe-/A2.
Microscopy for LNPs
Images were collected at the UBC High-Resolution Macromolecular Cryo-EM (HRMEM) facility on a 200kV Glacios microscope (Thermo Fisher) equipped with a Falcon 3 camera (Thermo Fisher) using TIA software. The magnification was 73kx, with a pixel size of 1.98A2. The dose was Xe-/A2, and the images were taken with a 3s exposure time and a nominal defocus of -2.5um.
Please notify the facility of your publications so we can feature you on the HRMEM website and Bluesky!
