EPU

  1. Do the beam alignments on a crossgrating or on carbon
  2. Check all the presets using the preview button and make sure the beam is aligned for all settings.
  3. Move over a hole to check the dose rate.
  4. Go to eucentric height and calibrate image shifts
  5. Check eucentric height and take an atlas
  6. Start a new session (check manual selection and choose the appropriate hole spacing)
  7. In Square selection choose a promising grid square and right click ‘add’ then ‘move stage to grid square’
  8. In AutoFunctions carry out ‘Auto-eucentric by beam tilt’ with the hole preset
  9. In Hole selection take a picture
  10. Use the ‘Measure hole size’ tool then click ‘Find holes’
  11. At this point if you want to be sure the square is good you can right click to move to a few holes (one in the middle, one at the edge) and take preview images with the ‘Data Acquisition’ preset to see if the particles look good
  12. Use ‘Filter Ice Quality’ to deselect holes that are too dark or too light
  13. Hold down shift and use the mouse to deselect holes manually
  14. Move to the ‘template definition tab’ and take a preview image
  15. Click ‘Find and Center hole’
  16. Click ‘add acquisition area’ and move it over the hole. Set the defocus values you want to collect at
  17. Click ‘Add AutoFocus area’ and move it on to the carbon. Change the delay after stage shift value to at least 10s.
  18. Click ‘Add Drift Measurement area’ and drag it over the autofocus area. Set the drift threshold to 0.08 and change the frequency to once per grid square
  19. Go to template execution and preview the acquisition
  20. Go back to square selection and add another square
  21. Move to the square and use AutoFunctions to set the eucentric height
  22. Go to hole selection, take a preview image, and select ‘Find holes’
  23. Manually refine the holes you want to collect on
  24. Repeat on as many squares as you want to collect
  25. Go to ‘Automated Acquisition’ and hit ‘Start run’