- Do the beam alignments on a crossgrating or on carbon
- Check all the presets using the preview button and make sure the beam is aligned for all settings.
- Move over a hole to check the dose rate.
- Go to eucentric height and calibrate image shifts
- Check eucentric height and take an atlas
- Start a new session (check manual selection and choose the appropriate hole spacing)
- In Square selection choose a promising grid square and right click ‘add’ then ‘move stage to grid square’
- In AutoFunctions carry out ‘Auto-eucentric by beam tilt’ with the hole preset
- In Hole selection take a picture
- Use the ‘Measure hole size’ tool then click ‘Find holes’
- At this point if you want to be sure the square is good you can right click to move to a few holes (one in the middle, one at the edge) and take preview images with the ‘Data Acquisition’ preset to see if the particles look good
- Use ‘Filter Ice Quality’ to deselect holes that are too dark or too light
- Hold down shift and use the mouse to deselect holes manually
- Move to the ‘template definition tab’ and take a preview image
- Click ‘Find and Center hole’
- Click ‘add acquisition area’ and move it over the hole. Set the defocus values you want to collect at
- Click ‘Add AutoFocus area’ and move it on to the carbon. Change the delay after stage shift value to at least 10s.
- Click ‘Add Drift Measurement area’ and drag it over the autofocus area. Set the drift threshold to 0.08 and change the frequency to once per grid square
- Go to template execution and preview the acquisition
- Go back to square selection and add another square
- Move to the square and use AutoFunctions to set the eucentric height
- Go to hole selection, take a preview image, and select ‘Find holes’
- Manually refine the holes you want to collect on
- Repeat on as many squares as you want to collect
- Go to ‘Automated Acquisition’ and hit ‘Start run’