Samples are prepared for cryo-EM by plunge freezing in liquid ethane. This results in rapid freezing of the water into vitreous ice containing the suspended samples.
Steps of the freezing process are:
- Application of the sample to a grid.
- Blotting of the sample
- Freezing in liquid ethane
The facility is equipped with an FEI Vitrobot for automated plunge freezing. Please bring your own grids which can be purchased through several suppliers.
See below for some helpful videos on sample preparation!